کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1243343 1495797 2015 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Electrokinetic acceleration of DNA hybridization in microsystems
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Electrokinetic acceleration of DNA hybridization in microsystems
چکیده انگلیسی


• In situ DNA hybridization was accelerated by local microfluidic vortexes.
• Hybridization efficiency was controlled by electrokinetic forces.
• DNA hybridization was completed as short as 5 min.
• 5-min Dynamic hybridization increased 4.5 fold of signal intensity.
• Dynamic hybridization showed better specificity than static hybridization.

In this work, electrokinetic acceleration of DNA hybridization was investigated by different combinations of frequencies and amplitudes of actuating electric signals. Because the frequencies from low to high can induce different kinds of electrokinetic forces, i.e., electroosmotic to electrothermal forces, this work provides an in-depth investigation of electrokinetic enhanced hybridization. Concentric circular Cr/Au microelectrodes of 350 µm in diameter were fabricated on a glass substrate and probe DNA was immobilized on the electrode surface. Target DNA labeled with fluorescent dyes suspending in solution was then applied to the electrode. Different electrokinetic forces were induced by the application of different electric signals to the circular microelectrodes. Local microfluidic vortexes were generated to increase the collision efficiency between the target DNA suspending in solution and probe DNA immobilized on the electrode surface. DNA hybridization on the electrode surface could be accelerated by the electrokinetic forces. The level of hybridization was represented by the fluorescent signal intensity ratio. Results revealed that such 5-min dynamic hybridization increased 4.5 fold of signal intensity ratio as compared to a 1-h static hybridization. Moreover, dynamic hybridization was found to have better differentiation ability between specific and non-specific target DNA. This study provides a strategy to accelerate DNA hybridization in microsystems.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 138, 1 June 2015, Pages 149–154
نویسندگان
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