کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1243372 | 969659 | 2008 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Fluorometric determination of heparin based on self-quenching of fluorescein-labeled protamine
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A new signaling technique for a fluorometric heparin assay has been developed using the self-quenching of fluoresceinisothiocyanate-labeled protamine (F-protamine). The binding between F-protamine and heparin led to a significant fluorescence quenching due to enhancing the proximity of the F-protamine molecules. The fluorescence of F-protamine (5.9 μg/mL) decreased to 13% in the presence of 2.0 μg/mL heparin. An advantage of this self-quenching system is that the detection can be carried out without using other indicators. With the present system, we could monitor the therapeutic level of heparin in bovine plasma, and the response was quick. These properties of the self-quenching system are suitable for practical use.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 76, Issue 4, 15 August 2008, Pages 736–741
Journal: Talanta - Volume 76, Issue 4, 15 August 2008, Pages 736–741
نویسندگان
Yuya Egawa, Ryosuke Hayashida, Toshinobu Seki, Jun-ichi Anzai,