کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1243529 | 969664 | 2008 | 5 صفحه PDF | دانلود رایگان |
A pentafluorophenylpropyl (PFPP) stationary phase was for the first time tested for the simultaneous determination of triamcinolone acetonide, its degradation product triamcinolone and two preservatives, methylparaben, and propylparaben. A new simple isocratic reversed phase HPLC method with UV detection, using estradiol hemihydrate as an internal standard, has been developed and validated. Chromatography was performed on a Discovery HS F5 column (150 mm × 4.6 mm, 5 μm) using a binary mobile phase composed of acetonitrile and water 45:55 (v:v). The flow-rate was 0.6 mL/min, the column temperature 25 °C and the UV detection was accomplished at 240 nm.The chromatography results using PFPP stationary phase were compared with those obtained using conventional C18 columns.
Journal: Talanta - Volume 76, Issue 3, 30 July 2008, Pages 597–601