کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1243672 | 969668 | 2009 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Multivariate optimization and validation of an analytical methodology by RP-HPLC for the determination of losartan potassium in capsules Multivariate optimization and validation of an analytical methodology by RP-HPLC for the determination of losartan potassium in capsules](/preview/png/1243672.png)
This paper describes the optimization and validation of an analytical methodology for the determination of losartan potassium in capsules by HPLC using 25-1 fractional factorial and Doehlert designs. This multivariate approach allows a considerable improvement in chromatographic performance using fewer experiments, without additional cost for columns or other equipment. The HPLC method utilized potassium phosphate buffer (pH 6.2; 58 mmol L−1)–acetonitrile (65:35, v/v) as the mobile phase, pumped at a flow rate of 1.0 mL min−1. An octylsilane column (100 mm × 4.6 mm i.d., 5 μm) maintained at 35 °C was used as the stationary phase. UV detection was performed at 254 nm. The method was validated according to the ICH guidelines, showing accuracy, precision (intra-day relative standard deviation (R.S.D.) and inter-day R.S.D values <2.0%), selectivity, robustness and linearity (r = 0.9998) over a concentration range from 30 to 70 mg L−1 of losartan potassium. The limits of detection and quantification were 0.114 and 0.420 mg L−1, respectively. The validated method may be used to quantify losartan potassium in capsules and to determine the stability of this drug.
Journal: Talanta - Volume 80, Issue 1, 15 November 2009, Pages 236–241