کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1244166 969681 2011 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Chemiluminescence detection of label-free C-reactive protein based on catalytic activity of gold nanoparticles
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Chemiluminescence detection of label-free C-reactive protein based on catalytic activity of gold nanoparticles
چکیده انگلیسی

A novel, quantitative analytical method for measuring C-reactive protein (CRP) levels in human serum has been developed based on the catalytic activity of gold nanoparticles (GNPs) and luminol–H2O2 chemiluminescence (CL). The CL intensity in the presence of CRP and its ligand, O-phosphorylethanolamine (PEA), was greatly enhanced due to the aggregation of GNPs after the addition of 0.5 M NaCl. Any pretreatment steps, such as covalent functionalization of GNPs, addition of antibodies, or labeling of CRP, were not needed for CL detection. The CL enhancement was linearly proportional to CRP concentration in the range of 1.88 fM to 1.925 pM. The detection limit of CRP in serum samples was estimated to be as low as 1.88 fM. The detection sensitivity was increased more than 164 times of magnitude over that of the conventional, enzyme-linked immunosorbent assay (ELISA) method. This proposed GNP-based CL detection method offers the advantages of simplicity, rapidity, and sensitivity.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 84, Issue 3, 15 May 2011, Pages 752–758
نویسندگان
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