کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1244219 | 969683 | 2007 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Total internal reflected resonance light scattering determination of protein in human blood serum at water/tetrachloromethane interface with Arsenazo-TB and Cetyltrimethylammonium bromide
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
A direct quantification of protein in human blood serum samples is proposed based on the measurement of total internal reflected resonance light scattering (TIR-RLS) at water/tetrachloromethane (H2O/CCl4) interfaces. At pH 4.10 and in the presence of Cetyltrimethylammonium bromide (CTAB), the interaction of Arsenazo-TB (ATB) with human serum albumin (HSA) results in strong enhanced TIR-RLS signals with the maximum spectra peak located at 330 nm. The enhanced TIR-RLS intensity at 330 nm was found to be proportional to the concentration of HSA in the range 0.5-42.3 μg/ml and its limit of determination (3Ï) is 198 ng/ml. The content of HSA in blood serum samples were determined with the recovery of 97-103% for standard addition experiment.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 71, Issue 2, 15 February 2007, Pages 555-560
Journal: Talanta - Volume 71, Issue 2, 15 February 2007, Pages 555-560
نویسندگان
Lijun Dong, Xingguo Chen, Zhide Hu,