کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1244345 | 969685 | 2010 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: On-line solid-phase extraction–HPLC–fluorescence detection for simultaneous determination of puerarin and daidzein in human serum On-line solid-phase extraction–HPLC–fluorescence detection for simultaneous determination of puerarin and daidzein in human serum](/preview/png/1244345.png)
Response surface methodology (RSM) was applied to the optimization of on-line solid-phase extraction (SPE) parameters, and an automated system of on-line SPE coupled with high-performance liquid chromatography (HPLC) with fluorescence detection was developed for the determination of puerarin and daidzein in human serum. The human serum sample of 50 μL was injected into a conditioned C18 SPE cartridge, and the matrix was washed out with acetonitrile–KH2PO4–triethylamine buffer (0.01 M, pH 7.4) (3:97, v/v) for 3 min at a flow rate of 0.25 mL/min. Then the target analytes were eluted and transferred to the analytical column. A chromatographic gradient elution was programmed with the mobile phase consisting of acetonitrile and KH2PO4–triethylamine buffer, and the analytes were determined with a fluorescence detector at excitation wavelength of 350 nm and emission wavelength of 472 nm, respectively. The proposed method presented good linear relations (0.85–170 μg/mL for puerarin and 0.2–40 μg/mL for daidzein), satisfactory precision (RSD < 8%), and accredited recovery (92.5–107.8%).
Journal: Talanta - Volume 82, Issue 4, 15 September 2010, Pages 1212–1217