کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1245216 969715 2009 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Phosphotriesterase: A complementary tool for the selective detection of two organophosphate insecticides: Chlorpyrifos and chlorfenvinfos
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Phosphotriesterase: A complementary tool for the selective detection of two organophosphate insecticides: Chlorpyrifos and chlorfenvinfos
چکیده انگلیسی

This work shows the possibility of combining the high sensitivity of genetically-modified Drosophila melanogaster acetylcholinesterase (B394) with the ability of phosphotriesterase (PTE) to hydrolyse organophosphate compounds, in the aim of developing a biosensor selective to two insecticides of interest: chlorpyrifos and chlorfenvinfos. The studies clearly demonstrate that chlorfenvinfos is a substrate that acts as competitive inhibitor of PTE, therefore preventing the efficient hydrolysis of other pesticides, including chlorpyrifos. A bi-enzymatic sensor was designed by immobilizing both B394 and PTE in a polyvinylalcohol matrix. The sensor was shown to be able to discriminate between chlorpyrifos and chlorfenvinfos inhibitions.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 77, Issue 5, 15 March 2009, Pages 1627–1631
نویسندگان
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