کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1245501 969724 2006 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Immobilization of streptavidin in sol–gel films: Application on the diagnosis of hepatitis C virus
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Immobilization of streptavidin in sol–gel films: Application on the diagnosis of hepatitis C virus
چکیده انگلیسی

Recent advances have accelerated the development of biosensors for the analysis of specific gene sequences. In this kind of biosensor, a DNA probe is immobilized on a transducer and the hybridization with the target DNA is monitored by suitable methodology. In the present work, the streptavidin (STA) was encapsulated in thin films siloxane–poly(propylene oxide) hybrids prepared by sol–gel method and deposited on the graphite electrode surface by dip-coating process. Biotinylated 18-mer probes were immobilized through STA and a novel amperometric DNA biosensor for the detection and genotyping of the hepatitis C virus (genotypes 1, 2A/C, 2B and 3) is described. The HCV RNA from serum was submitted to reverse transcriptase-linked polymerase chain reaction (RT-PCR) and biotin-labeled cDNA was obtained. Thus, the cDNA was hybridized to the target-specific oligonucleotide probe immobilized on the graphite electrode surface and following the avidin–peroxidase conjugate was added. The enzymatic response was investigated by constant potential amperometry at −0.45 V versus Ag/AgCl using H2O2 and KI solutions. HCV RNA negative and positive controls and positive samples of sera patients were analyzed and the results were compared to commercial kit. The proposed methodology appeared to be suitable and convenient tool for streptavidin immobilization and diagnose of HCV disease.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 70, Issue 3, 15 October 2006, Pages 637–643
نویسندگان
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