کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1245678 | 969729 | 2006 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Determination of carbofuran in water by homogeneous immunoassay using selectively conjugate mastoparan and terbium/dipicolinic acid fluorescent complex
دانلود مقاله + سفارش ترجمه
دانلود مقاله ISI انگلیسی
رایگان برای ایرانیان
کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Homogeneous immunoassay (LITRFIA) for carbofuran (CF) determination was performed using liposomes and mastoparan (Mast) conjugate as cytolitic agent. Mast was conjugated to the 5-(2-2-dimethyl-2,3-dihydro-benzofuran-7-yloxy)-pentanoic acid (CPCF) both randomly and selectively to a single (V1- or K4-) amino-group. The conjugated compounds have been tested for the cytolytic activity on liposomes trapping Tb/citrate complex. Dipicolinic acid (DPA) was used as fluorescent chelating agent. The CPCF-V1-Mast derivative (retaining almost the same lytic activity as Mast) was used in the immunoassay in competition with standard CF. Liposome lysis was proportional to the standard concentrations in a dynamic range between 10Â pg and 10Â ng. Assay has been performed for tap water analysis and for 10 real samples taken from an agricultural area to the south of Milan. Recovery in samples spiked with two different CF concentrations was between 92.5 and 105%.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 69, Issue 5, 15 July 2006, Pages 1106-1111
Journal: Talanta - Volume 69, Issue 5, 15 July 2006, Pages 1106-1111
نویسندگان
Maria Angela Bacigalupo, Giacomo Meroni, Renato Longhi,