کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1246169 969746 2007 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Development and validation of a HPLC method for the determination of voriconazole in pharmaceutical formulation using an experimental design
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Development and validation of a HPLC method for the determination of voriconazole in pharmaceutical formulation using an experimental design
چکیده انگلیسی

A rapid and sensitive RP–HPLC method with UV detection (260 nm) for routine analysis of voriconazole in a pharmaceutical formulation (Vfend®) was developed. Chromatography was performed with mobile phase containing a mixture of acetonitrile and water (50:50, v/v) with flow rate was of 1.0 ml min−1. Quantitation was accomplished with internal standard method. The procedure was validated for linearity (correlation coefficient = 0.9999), accuracy, robustness and intermediate precision. Experimental design was used for validation of robustness and intermediate precision. To test robustness, three factors were considered. Percentage of acetonitrile in mobile phase, flow rate and pH; an increase in the flow rate results in a decrease of the drug found concentration, while the percentage of organic modifier and pH have no important effect on the response. For intermediate precision measure the variables considered were: analyst, equipment and number of days. The R.S.D. value (0.45%, n = 24) indicated a good precision of the analytical method. The proposed method was simple, highly sensitive, precise and accurate and retention time less than 4 min indicating that the method is useful for routine quality control.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 71, Issue 3, 28 February 2007, Pages 1424–1429
نویسندگان
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