کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1246410 969754 2006 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
FTIR-determination of sterols from the red alga Asparagopsis armata: Comparative studies with HPLC
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
FTIR-determination of sterols from the red alga Asparagopsis armata: Comparative studies with HPLC
چکیده انگلیسی

An analytical procedure was developed for the determination of the total amount of sterols in the red alga Asparagopsis armata, globally determined as cholesterol, which is the major sterol contained in red algae. Samples, previously saponified with KOH were preconcentrated on DSC-18 solid phase cartridges (SPE) and eluted with dichloromethane stabilized with β-amylene. Fourier transform infrared (FTIR) spectrometry was employed for selective detection at 1049 cm−1 with a baseline established between 1000 and 1079 cm−1. The results were compared to those obtained by high performance liquid chromatography (HPLC). The concentration obtained in actual samples from alga was 3.37% (w/w) by FTIR and 3.30% (w/w) by HPLC, showing a good comparability between the two methods.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 68, Issue 4, 15 February 2006, Pages 1230–1235
نویسندگان
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