Electro-oxidation of glucose at self-assembled monolayers incorporated by copper particles

A novel copper incorporated self-assembled monolayers (SAMs) modified gold electrode (Cu/SAMs) for determination of glucose was developed by electrodepositing Cu particles on the SAMs of hexanethiol. The scanning electron microscopic (SEM) images showed that copper formed orbicular particles of nanosizes on the SAMs, which was much different from the fractal-like particles of copper formed at gold electrode. The Cu/SAMs film electrode exhibited high sensitivity to glucose oxidation and depressed responses towards some interferents of glucose in blood like uric acid and ascorbic acid. Under optimal working conditions, the oxidation current of glucose was proportional to the concentration of glucose in the range from 3.0 μM to 10 mM by amperometry with a low detection limit of 0.7 μM glucose (S/N = 3). This electrode was successfully applied to the determination of glucose in rat blood and the results were satisfactory.