کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1246889 | 1495853 | 2009 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Investigation of voltammetric enzyme-linked immunoassay system based on N-heterocyclic substrate of 2,3-diaminopyridine
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
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چکیده انگلیسی
A new voltammetric enzyme-linked immunoassay system using the electrochemical substrate 2,3-diaminopyridine (DAP) and horseradish peroxidase (HRP) system has been developed. DAP is oxidized with H2O2 catalyzed by HRP, and the resulting electroactive product produces a sensitive voltammetric peak at potential of â0.72 V (vs. saturated calomel electrode (SCE)) in Britton-Robinson (BR) buffer solutions. The enzyme-catalyzed reaction conditions and voltammetric detection conditions have been investigated in detail. Under the selected optimum conditions, the linear range for detection of free HRP is from 6.0 Ã 10â11 to 1.0 Ã 10â8 g mLâ1 with a detection limit of 1.0 Ã 10â12 g mLâ1. The new voltammetric detection system has been successfully applied for the assay of prostate specific antigen (PSA) in human serum ranging from 0.4 to 100 ng mLâ1 with a detection limit of 0.1 ng mLâ1, which is five times lower than that of traditional o-phenylenediamine (OPD) spectrophotometric enzyme-linked immunosorbent assay (ELISA) method. The proposed N-heterocyclic electrochemical detection system of DAP-H2O2-HRP has provided a new and much improved immunoassay method.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 78, Issues 4â5, 15 June 2009, Pages 1395-1400
Journal: Talanta - Volume 78, Issues 4â5, 15 June 2009, Pages 1395-1400
نویسندگان
Fengli Yu, Ping Du, Xi Lei, Shusheng Zhang,