کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1247300 969818 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Organic–inorganic matrix for electrochemical immunoassay: Detection of human IgG based on ZnO/chitosan composite
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Organic–inorganic matrix for electrochemical immunoassay: Detection of human IgG based on ZnO/chitosan composite
چکیده انگلیسی

A new strategy to construct amperometric immunosensor for human IgG assay based on ZnO/chitosan composite as sensing platform has been described. This material, which combined the advantages of inorganic species, ZnO and organic polymer, chitosan, can maintain biological activity well. A sequential sandwich immunoassay format was performed on the ZnO/chitosan composite supported by glass carbon electrode (GCE) using goat-anti-human IgG antibody (IgG Ab) and human IgG as a model system. Amperometry was used to determine the amount of horse-radish peroxidase (HRP) fixed on the sensor surface, which was related to the content of the desired human IgG. Assay conditions that were optimized included the amount of labeled antibody, the incubation time and temperature, the pH of the substrate solution, etc. Using hydroquinone as a mediator, amperometric detection at −150 mV (versus SCE) resulted in a detection range 2.5–500 ng mL−1, with a detection limit of 1.2 ng mL−1. The simple manipulations of the construction of ZnO/chitosan composite, as well as low-cost and broad linear range, are the main features of the proposed immunosensing method.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 69, Issue 3, 15 May 2006, Pages 686–690
نویسندگان
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