کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1247345 | 969824 | 2006 | 6 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Development of an on-line preconcentration system for zinc determination in biological samples
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Development of an on-line preconcentration system for zinc determination in biological samples Development of an on-line preconcentration system for zinc determination in biological samples](/preview/png/1247345.png)
چکیده انگلیسی
An on-line preconcentration system for zinc determination in 24-h urine, blood plasma and erythrocyte matrices by flame atomic absorption spectrometry (FAAS) was used. This procedure was based on adsorption of Zn(II) ions onto a minicolumn filled with silica gel, chemically modified with niobium(V) oxide (Nb2O5-SiO2). The determination of the optimum conditions for Zn(II) preconcentration was done using two-level full factorial and Doehlert designs. In the optimization procedure, four variables (sample pH, eluent concentration, sample flow rate and eluent flow rate) were investigated. The results obtained from the full factorial design demonstrated that the sample pH and sample flow rate variables, and their interactions, were statistically significant. A Doehlert matrix was used in order to determine the optimum conditions for the sample pH and sample flow rate. The optimized conditions for sample pH and flow rate sampling were 6.6 and 7.1 ml minâ1, respectively, to obtain the maximum Zn(II) preconcentration and determination in the biological samples studied. Parameters of analytical curve, precision, effect of other ions in the proposed system and accuracy were achieved to assess the proposed method. The accuracy was confirmed by analysis of certified reference materials (urine Seronorm⢠Trace Elements) and recovery tests in blood plasma and erythrocyte samples. Detection limit (3Ï/S) of 0.77 μg lâ1, precision (calculated as relative standard deviation) of 1.5% for Zn(II) concentration of 10 μg lâ1 (n = 7) and a sampling frequency of 27 samples/h were obtained from the proposed system.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Talanta - Volume 69, Issue 2, 15 April 2006, Pages 488-493
Journal: Talanta - Volume 69, Issue 2, 15 April 2006, Pages 488-493
نویسندگان
Rosilene L. Dutra, Heloisa F. Maltez, Eduardo Carasek,