Peak measurement and calibration in chromatographic analysis

• Data acquisition and processing techniques in chromatography are discussed.
• Topical issues of the theory and practice of calibration are elucidated.
• Nonlinear calibration models are considered.
• Recommendations are given to design and perform calibration properly.

The precision and accuracy of quantitative chromatographic analysis depends significantly on the quality of calibration. This review considers such aspects of the calibration procedure as design of a calibration experiment, choice of a calibration model, and regression analysis of calibration data. A brief description of the theory of chromatographic detection and modern approaches to data acquisition and processing is given in the context of the calibration problem.