کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1247844 | 1495903 | 2014 | 15 صفحه PDF | دانلود رایگان |
• State of the art in LC-MS-based lipidomics.
• Sample extraction, separation, ionization and detection in LC-MS-based lipidomics.
• Data processing, lipid identification/quantification, quality control in lipidomics.
• Highlights of recent lipidomics studies.
Liquid chromatography-mass spectrometry (LC-MS)-based lipidomics has undergone dramatic developments over the past decade. This review focuses on state of the art in LC-MS-based lipidomics, covering all the steps of global lipidomic profiling.By reviewing 185 original papers and application notes, we can conclude that current advanced LC-MS-based lipidomics methods involve:(1)lipid extraction schemes using chloroform/MeOH or methyl tert-butyl ether (MTBE)/MeOH, both with addition of internal standards covering each lipid class;(2)LC separation of lipids using short microbore C18 or C8 columns with sub-2-µm or 2.6–2.8-µm (fused-core) particle size with analysis time <30 min;(3)electrospray ionization in positive- and negative-ion modes with full spectra acquisition using high-resolution MS with capability to MS/MS.Phospholipids (phosphatidylcholines, phosphatidylethanolamines, phosphatidylinositols, phosphatidylserines, phosphatidylglycerols) followed by sphingomyelins, di- and tri-acylglycerols, and ceramides were the most frequently targeted lipid species.
Journal: TrAC Trends in Analytical Chemistry - Volume 61, October 2014, Pages 192–206