کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1248334 1495934 2011 11 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Sample preparation and current applications of liquid chromatography for the determination of non-structural carbohydrates in plants
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Sample preparation and current applications of liquid chromatography for the determination of non-structural carbohydrates in plants
چکیده انگلیسی

This article summarizes the current methods of determination of non-structural carbohydrates (NSCs) in plant samples based on liquid chromatography (LC). NSCs comprise several types of carbohydrates: sugar alcohols (e.g., sorbitol), monosaccharides (e.g., glucose and fructose), disaccharides (e.g., sucrose), oligosaccharides (e.g., raffinose) and polysaccharides [e.g., starch and polyfructans (e.g., inulin)]. NSCs are important in plant metabolism and have to be strictly distinguished from all sorts of structural carbohydrates (e.g., polysaccharide cellulose) that make up the backbone of the plants. Consequently, preservation of structural carbohydrates is a crucial step during sample preparation for NSC determination and is therefore addressed.Sugar alcohols, monosaccharides, disaccharides and those oligosaccharides that are easily soluble in polar solvents can be analyzed directly by high-performance LC. They are also referred to as free carbohydrates (FCs).However, polysaccharides are generally submitted to hydrolyzation into monomers prior to their quantitative analysis. This can be done either chemically, using acids, or enzymatically – both methods are discussed. For identification and quantification of the NSCs after LC separation, the following detectors are used: pulsed amperometry, refractive index, evaporate light scattering and finally, mass spectrometry.


► This article reviews the analysis of non-structural carbohydrates (NSC) by HPLC.
► Review includes sample preparation techniques.
► HPLC is coupled to various detectors: PAD, RID, ELSD and MS.
► High analytical versatility allows application to various aspects of NSC analyses.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: TrAC Trends in Analytical Chemistry - Volume 30, Issue 11, December 2011, Pages 1833–1843
نویسندگان
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