کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1248500 | 970439 | 2007 | 13 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Visualizing chemical interactions in life sciences with wide-field fluorescence microscopy towards the single-molecule level
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
چکیده انگلیسی
Systematic understanding of dynamic biological networks is grounded at the molecular level. Knowledge at this level will discover the functions of molecular components and their interactions with cells or among the molecules themselves. Single-molecule detection has proved to be an indispensable technique for characterizing molecular interactions in various physiological conditions. Among the single-molecule-detection methods, wide-field microscopy-based fluorescence imaging is unique in that dynamic single-molecule events can be vividly observed in a wide observation field. Such a technique has obvious advantages for direct tracking of the pathways and processes of molecular interactions and giving visual information about the molecular mechanisms of many biological events. We discuss experimental schemes and applications of wide-field fluorescence microscopy in characterizing biological molecular interactions and studying virus-cell or particle-cell interactions.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: TrAC Trends in Analytical Chemistry - Volume 26, Issue 10, November 2007, Pages 980-992
Journal: TrAC Trends in Analytical Chemistry - Volume 26, Issue 10, November 2007, Pages 980-992
نویسندگان
Hongwei Gai, Isaac Stayton, Xin Liu, Bingcheng Lin, Yinfa Ma,