کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1249495 1495973 2016 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Rapid detection of Pseudomonas aeruginosa by phage-capture system coupled with micro-Raman spectroscopy
ترجمه فارسی عنوان
تشخیص سریع سودوموناس آئروژینوزا با سیستم ضبط فاژ و همراه با طیف سنجی میکرو رامان
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
چکیده انگلیسی


• Early and accurate identification of medically relevant microorganisms like Pseudomonas aeruginosa.
• Phage display is a high-throughput biotechnique for selection of specific molecular.
• Raman and FTIR spectroscopic techniques allow to identify molecules vibrating modes in bacteria.

The early and accurate identification of medically relevant microorganisms like Pseudomonas aeruginosa is of great importance for human health. Current pathogen identification is routinely performed using conventional microbiological methods which are unable to detect fastidious organism that are difficult to culture and occur at low concentrations without time consuming multiple cultivation steps. This study reports the development of a novel rapid and cultivation-free method for highly sensitive and rapid detection of Pseudomonas aeruginosa based on the coupling of phage-capture system with optical techniques, namely FTIR and visible micro-Raman spectroscopies. Commercial latex beads were functionalized with the entire structure of engineered phage clones and used as bacterial capture and concentrating system. The rapid concentration of bacteria enhanced the detection of the Raman scattering signal by increasing the location concentration that is being processed. This method can be used to detect a low level of P. aeruginosa (103 cells/ml) from clinical samples without the use of selective media or additional biochemical tests. The sample testing process, including data acquisition, required a time less than one hour. The proposed system represents a proof of concept study for development of sensitive phage-based biosensors for rapid and specific one-step detection of pathogenic bacteria.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vibrational Spectroscopy - Volume 86, September 2016, Pages 1–7
نویسندگان
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