کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1249851 970743 2006 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Raman optical activity of the hinge peptide
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آنالیزی یا شیمی تجزیه
پیش نمایش صفحه اول مقاله
Raman optical activity of the hinge peptide
چکیده انگلیسی
We report results of Raman optical activity investigation of the hinge peptide (a parallel dimer of the octapeptide H-Thr-Cys-Pro-Pro-Cys-Pro-Ala-Pro-OH with two disulphide bonds) derived from the sequence of human IgG1, its single thread model (a linear octapeptide H-Thr-Met-Pro-Pro-Met-Pro-Ala-Pro-OH), S-S bridged tetrapeptide (H-Gly-Cys-OH)2 and poly-l-proline. The ROA spectra confirm PPII as a prevailing conformation of the double thread hinge peptide in aqueous solution while for its single thread Met analog PPII conformation as the only conformation. Diagnostic positive ROA band at 1320 cm−1 remains present even in proline-rich peptides and can serve as reliable PPII marker. Significant positive ROA signal related to C-S stretching vibration has been found but a clear ROA signature of S-S stretching mode still remains uncertain.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Vibrational Spectroscopy - Volume 42, Issue 1, 18 October 2006, Pages 88-92
نویسندگان
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