کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1251825 | 1495987 | 2014 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Multiple detection of proteins by SERS-based immunoassay with core shell magnetic gold nanoparticles Multiple detection of proteins by SERS-based immunoassay with core shell magnetic gold nanoparticles](/preview/png/1251825.png)
A highly selective and sensitive surface-enhanced Raman scattering (SERS)-based immunoassay for the multiple detection of proteins has been developed. The proposed core shell magnetic gold (Au) nanoparticles allow for successful protein separation and high SERS enhancement for protein detection. To selectively detect a specific protein in a mixed protein solution, we employed the sandwich type SERS immunoassay with core shell magnetic Au nanoparticles utilizing specific antigen–antibody interactions. Based on this proposed SERS immunoassay, we can successfully detect proteins in very low concentrations (∼800 ag/mL of mouse IgG and ∼5 fg/mL of human IgG) with high reproducibility. Magnetically assisted protein separation and detection by this proposed SERS immunoassay would provide great potential for effective and sensitive multiple protein detection. This technique allows for the straightforward SERS-based bioassays for quantitative protein detections.
Journal: Vibrational Spectroscopy - Volume 72, May 2014, Pages 44–49