Improved method to evaluate the ability of compounds to destabilize the cellular plasma membrane

► The modified erythrocyte osmotic fragility measurement is proposed. ► Improvement of the method precision by the controlling the final level of hemolysis. ► Reduction of intra- and inter-sample variations. ► The membrane disturbing proteins at non-hemolytic concentrations do not affect plasma membrane mechanics. ► There are experimentally significant differences between human and rabbit erythrocytes.