Immobilization of laccase by Cu2+ chelate affinity interaction on surface modified magnetic silica particles and its use for the removal of pentachlorophenol

Magnetic Cu2+-chelated silica particles using polyacrylamide as a metal-chelating ligand was developed and used for the immobilization of laccase by coordination. The effect of pH and temperature on the enzymatic property of immobilized laccase and its catalytic capacity for pentachlorophenol (PCP) degradation were evaluated systemically. Compared with free laccase, the immobilized laccase showed improved acid adaptability and thermal stability. The immobilized laccase prepared in this work exhibited a good catalytic capacity for PCP removal from aqueous solutions.