کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1272752 972564 2006 8 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Differential pulse voltammetric studies of ethidium bromide binding to DNA
موضوعات مرتبط
مهندسی و علوم پایه شیمی الکتروشیمی
پیش نمایش صفحه اول مقاله
Differential pulse voltammetric studies of ethidium bromide binding to DNA
چکیده انگلیسی

The interaction of ethidium bromide (EtBr) with calf thymus DNA is investigated electrochemically with the use of differential pulse voltammetry (DPV) at two different ionic strengths of a solution (0.154 M and 0.02 M [Na+], pH 7.0). It is revealed that EtBr binds with DNA in more than one way. The appropriate values of constants (K) and number site sizes (n) of EtBr binding to DNA are determined. The values of binding constants are equal to 1.9 · 106 and 5.6 · 105 M− 1, and number site sizes to 9 and 3.6 for strong interactions at ionic strengths of solutions 0.02 and 0.154 M Na+ at 28 °C, respectively. For a weaker interaction, these parameters are equal to 7 · 104 and 8 · 104 M− 1 and 1.5 and 1 at the mentioned ionic strengths of solutions, respectively. Thus, EtBr interacts with DNA in more than one way—intercalative and electrostatic at low ionic strength, and semi-intercalative and electrostatic at a higher strength of the solution. These results are in good accordance with the ones obtained by spectroscopic (absorption and fluorimetric) methods.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioelectrochemistry - Volume 68, Issue 1, January 2006, Pages 48–55
نویسندگان
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