کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1274459 1496925 2012 6 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Complexation of cytochrome c with calixarenes incorporated into the lipid vesicles and supported membranes
موضوعات مرتبط
مهندسی و علوم پایه شیمی الکتروشیمی
پیش نمایش صفحه اول مقاله
Complexation of cytochrome c with calixarenes incorporated into the lipid vesicles and supported membranes
چکیده انگلیسی

We studied the interaction of cytochrome c (cyt c) with specific calixarenes (CX) incorporated into the large unilamellar vesicles (LUV) composed of dimyristoylphosphatidylcholine (DMPC) or supported lipid membranes (sBLM) and compared this with not specific adsorption of cyt c to the LUV containing DMPC and anionic phosphatidic acid (PA) or sBLM composed of a mixture of DMPC and dimyristoylphosphatidic acid (DMPA). We showed that with increasing concentration of CX the average size of LUV increased and zeta potential become more negative as it is suggested from dynamic light scattering experiments. For PA containing LUV the increase in vesicle diameter was less expressed, but zeta potential decreased similarly like that of LUV contained CX. Cyt c did not affect significantly the LUV size, but reduced the negative zeta potential both for CX and PA containing vesicles. Electrochemical impedance spectroscopy allowed us to determine binding of cyt c to sBLM contained CX or DMPA. In both cases we observed decrease of charge transfer resistance with increasing cyt c concentration. The analysis of binding process suggests that the main driving force for interaction of cyt c with sBLM is the negative surface charge.

Figure optionsDownload as PowerPoint slideHighlights
► Calixarenes (CX) affect the size and zeta potential of lipid vesicles.
► Cytochrome c (cyt c) reduces the negative charge of vesicles, but does not affect their size.
► Cyt c reduces the charge transfer resistance at electrodes coated with supported membranes containing CX or negatively charged lipids.
► Negative surface charge is the main driving force in interaction of cyt c with membranes.

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioelectrochemistry - Volume 87, October 2012, Pages 220–225
نویسندگان
, , , , ,