کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1274564 | 1497549 | 2012 | 7 صفحه PDF | دانلود رایگان |
Biohydrogen production from untreated rice straw using different heat-treated sludge, initial cultivation pH, substrate concentration and particle size was evaluated at 55 °C. The peak hydrogen production yield of 24.8 mL/g TS was obtained with rice straw concentration 90 g TS/L, particle size <0.297 mm and heat-treated sludge S1 at pH 6.5 and 55 °C in batch test. Hydrogen production using sludge S1 resulted from acetate-type fermentation and was pH dependent. The maximum hydrogen production (P), production rate (Rm) and lag (λ) were 733 mL, 18 mL/h and 45 h respectively. Repeated-batch operation showed decreasing trend in hydrogen production probably due to overloading of substrate and its non-utilization. PCR-DGGE showed both hydrolytic and fermentative bacteria (Clostridium pasteurianum, Clostridium stercorarium and Thermoanaerobacterium saccharolyticum) in the repeated-batch reactor, which perhaps in association led to the microbial hydrolysis and fermentation of raw rice straw avoiding the pretreatment step.
► Peak HY of 25 mL H2/g TS under batch test was obtained at initial pH 6.5 and 55 °C.
► Hydrogen production was pH dependent using heat-treated sludge S1.
► The main soluble metabolic product was acetate.
► Substrate overloading led to H2 production inhibition in repeated-batch system.
Journal: International Journal of Hydrogen Energy - Volume 37, Issue 20, October 2012, Pages 15540–15546