کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1275916 | 1497542 | 2013 | 13 صفحه PDF | دانلود رایگان |
Enriched activated sludge that can effectively convert crude glycerol into bio-hydrogen was selected by an eco-biotechnological approach, in very strict conditions, using biodiesel-derived glycerol as the only carbon source. The thus obtained functional consortium was characterized by the genera Klebsiella, Escherichia/Shigella and Cupriavidus. During enrichment, the dominant metabolic end-product shifted from a 1,3 propanediol to ethanol, with a concomitant increase of the hydrogen yield from 0.18 ± 0.003 to 0.66 ± 0.06 mol/mol and an almost five-fold increase of the hydrogen production. Glycerol degradation efficiency showed an increase of around 50%. In optimized and upscaled conditions it was possible to obtain a hydrogen production rate of 2960 mL H2/L/day ± 185 at a near stoichiometric yield (of 0.90 mol/mol ± 0.01), with a carbon recovery of almost 90%, both in sterile and non-sterile conditions. Glycerol was almost totally degraded (degradation efficiency of 97.42% ± 0.98), independently of the glycerol type used.
► Possibility to select bacteria directly on the crude glycerol.
► Efficient biological conversion of crude glycerol from biodiesel stream.
► High H2 yield and production, both on pure and crude glycerol.
► High yields also in non-sterile conditions.
► Importance of metabolic cooperation mechanisms (syntrophism and/or cross-feeding).
Journal: International Journal of Hydrogen Energy - Volume 38, Issue 3, 6 February 2013, Pages 1319–1331