کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1279137 | 1645388 | 2011 | 9 صفحه PDF | دانلود رایگان |
Extreme-thermophilic biohydrogen production from distillery wastewater was investigated in batch and continuous-mode operation. Hydrogen-producing mixed culture was enriched by repeated batch cultivations. Effect of temperature and pH on biohydrogen yield was investigated in batch experiments. The highest hydrogen yield of 196. mL/g-volatile solidsaddded (VSadded) was obtained at 70 °C and pH 7.0 in batch culture. Continuous biohydrogen production was performed in CSTR reactor with yield of 172.0 mL/g-VSadded at HRT (hydraulic retention time) of 4 days. The main metabolic products were acetate, lactate, and ethanol. Community structure of hydrogen-producing microflora was investigated by 16S rRNA gene sequence analysis. The microorganisms involved in both batch and continuous-mode operation were similar and hydrogen production was carried out by a group of extreme-thermophilic bacterial species related to Thermotoga, Coprothermobacter, Caldanaerobacter, Thermobrachium, and Caldicellulosiruptor.
► Biohydrogen production from distillery wastewater was investigated and hydrogen yield of 172.0 mL/g-VSadded was obtained in CSTR at 70 °C.
► Hydrogen-producing mixed culture was enriched by repeated batch cultivations.
► Bacterial species related to Thermotoga and Coprothermobacter species were found to be the dominant bacteria in the mixed cunture.
Journal: International Journal of Hydrogen Energy - Volume 36, Issue 14, July 2011, Pages 8243–8251