کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1316169 | 976431 | 2010 | 7 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
A mass spectrometric comparison of the interactions of cisplatin and transplatin with myoglobin
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی معدنی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: A mass spectrometric comparison of the interactions of cisplatin and transplatin with myoglobin A mass spectrometric comparison of the interactions of cisplatin and transplatin with myoglobin](/preview/png/1316169.png)
چکیده انگلیسی
Mass spectrometric studies of the interactions of cisplatin and transplatin with myoglobin (Mb) provide information concerning interaction kinetics, Mb adduct identity, and cisplatin and transplatin binding sites on Mb. Although the Mb-cisplatin interaction is faster than the Mb-transplatin interaction, monoadducts and diadducts were formed in both the interactions over 30Â h. In order to locate the binding sites of cisplatin and transplatin on Mb, digests of free Mb, Mb-cisplatin and Mb-transplatin adducts were subjected to analysis by Fourier transform mass spectrometry (FT-MS). This analysis revealed that two fragment ions, 1313.275+ and 1316.685+, were obtained only from the Mb-cisplatin and Mb-transplatin adduct digests. Tandem mass spectrometry (MS/MS and MS3) of the 1313.275+ and 1316.685+ ions indicate that these ions arise from [Pt(NH3)]2+ and [Pt(NH3)2]2+, respectively, bound to peptide His97-Gly153. The product-ion spectra of the MS/MS and MS3 analyses of the 1313.275+ ion indicate a common binding site of cisplatin and transplatin on His116-His119 residues. The interactions of cisplatin and transplatin with a dipeptide His-Ser and the three dimensional (3D) structure of native Mb suggest that cisplatin and transplatin coordinate to His116 and His119.
ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Inorganic Biochemistry - Volume 104, Issue 2, February 2010, Pages 186-192
Journal: Journal of Inorganic Biochemistry - Volume 104, Issue 2, February 2010, Pages 186-192
نویسندگان
Ting Zhao, Fred L. King,