کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1316820 | 976484 | 2009 | 5 صفحه PDF | دانلود رایگان |

Peroxynitrite (ONOO−/ONOOH), the product of the diffusion controlled reaction between nitric oxide (NO) and superoxide anion (O2-), is a strong oxidizing and nitrating agent. Several heme proteins react rapidly with peroxynitrite, some of them catalyze its decomposition. In this work we found, contrary to previous reports, that catalase, a ferriheme enzyme, catalytically scavenges peroxynitrite. The second-order reaction rate constants of peroxynitrite decay catalyzed by catalase increase with decreasing pH and are equal to (2.7 ± 0.2) × 106, (1.7 ± 0.1) × 106 and (0.8 ± 0.1) × 106 M−1 s−1 at pH 6.1, 7.1 and 8.0, respectively. This dependence suggests that peroxynitrous acid, ONOOH, is the species that reacts with heme center of catalase. The possible reaction mechanisms of the decay of peroxynitrite catalyzed by catalase and physiological relevance of this reaction are discussed.
Journal: Journal of Inorganic Biochemistry - Volume 103, Issue 10, October 2009, Pages 1375–1379