کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1317594 | 1499462 | 2013 | 8 صفحه PDF | دانلود رایگان |
• Formation of Cbl(III/II) and Cbi(III/II) thiocyanate complexes was probed.
• Linkage isomerism is feasible on both Co(III) and Co(II) centers.
• Hexacoordinated Cbl(II)–SCN complex is observed at high ligand concentrations.
• Thiocyanates do not interfere cyanide binding by Cbi(III).
The interaction of Co(III) and Co(II) cobalamin (Cbl) and cobinamide (Cbi) with thiocyanate was examined with UV–vis and EPR spectra. S/N-linkage isomerism was explored on Co(III) and Co(II) Cbl and Cbi models using density functional theory (DFT; BP86, B3LYP). Performed calculations suggest the prevalence of isothiocyanato isomers over thiocyanato complexes on both Co(III) and Co(II) centers. The formation of Cbl(II) complex with thiocyanate was observed at high ligand concentrations which was proposed to be hexacoordinated. DFT data maintain the possibility of hexacoordinated Co(II) complexes with thiocyanate in which one of extra-ligands is weakly coordinated. It is found that high thiocyanate concentrations could retard cyanide binding to cobalamin but not to cobinamide.
UV–vis, EPR, and DFT data on Cbl(II)-SCN− complex.Figure optionsDownload as PowerPoint slide
Journal: Journal of Inorganic Biochemistry - Volume 125, August 2013, Pages 32–39