کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1317922 976604 2011 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Inductive properties of polypyridyl ruthenium complexes significantly regulate various protein distributions in Escherichia coli
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی معدنی
پیش نمایش صفحه اول مقاله
Inductive properties of polypyridyl ruthenium complexes significantly regulate various protein distributions in Escherichia coli
چکیده انگلیسی

Ruthenium complexes with similar octahedral structures but different intrinsic inductive properties significantly influence the total cellular protein distributions, which may affect different metabolic pathways. A systematic study of the relationship between ruthenium complexes and Escherichia coli was undertaken, using two-dimensional gel electrophoresis analysis and the identification of various proteins by mass data mining. Based on the low similarities (< 40%) between the total protein distributions, the inductive properties of the ruthenium complexes are relevant to the formation of the protein–Ru interaction in addition to the Ru–DNA interaction. Two major protein functions in E. coli BL21 that were reduced by compound 1 were oxidoreductases and transporters, corresponding to 29% and 25% of the 24 down-regulated proteins. The main biological processes of the proteins down-regulated by compound 1 were related to carbohydrate reactions, including in transport, tricarboxylic acid (TCA) cycle, glycolysis, and gluconeogenesis. All four ruthenium complexes shared similar up-regulated proteins, including clpB and kpyk1, and down-regulated similar proteins, including ompA and ybbN. This result supports that the presence of Ru–protein interactions is a major factor affecting bacteria growth, and particularly transport and carbohydrate-related reactions.

Ruthenium complexes with similar octahedral structures but different inductive properties significantly influence the Ru–protein interactions, which may affect different metabolic pathways.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Inorganic Biochemistry - Volume 105, Issue 6, June 2011, Pages 902–910
نویسندگان
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