کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1317943 976615 2010 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The heme pocket of the globin domain of the globin-coupled sensor of Geobacter sulfurreducens — An EPR study
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی معدنی
پیش نمایش صفحه اول مقاله
The heme pocket of the globin domain of the globin-coupled sensor of Geobacter sulfurreducens — An EPR study
چکیده انگلیسی

The globin-coupled sensor (GCS) of Geobacter sulfurreducens is unique amongst GCSs in that its signalling domain is a transmembrane domain with yet unknown function. In the present work we use X-band continuous-wave and pulsed electron paramagnetic resonance (EPR) to investigate the ferric form of the globin domain of the G. sulfurreducens GCS (GsGCS162) at pH 8.5. This form shows a unique bis-histidine coordination of the heme with the F8His and E11His. In contrast with previous crystal structure data, where three conformers of the heme structure were identified, ferric GsGCS162 assumes only one conformation in frozen solution. The EPR data of ferric GsGCS162 are compared in detail with those of other bis-histidine coordinated globins, including other GCS systems.

EPR reveals a single conformer of the heme pocket in the globin domain of the globin-coupled sensor of G. sulfurreducensFigure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Journal of Inorganic Biochemistry - Volume 104, Issue 10, October 2010, Pages 1022–1028
نویسندگان
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