Trichophyton Soudanense and Trichophyton Mentagrophyte-treated Rice Husk Biomass Components and Effect of Yeast on the Bioethanol Yield

Trichophyton soudanense and Trichophyton mentagrophyte were isolated from the rice mill environment, cultured, and used singly and as co-culture in the treatment of measure quantities of pre-heated rice husk. Carboxymethyl cellulase activity of 57.61 μg/ml/min was optimum for T. mentagrophyte-treated rice husk crude enzymes at 50 °C and 80 °C, respectively. The duration of 120 h gave the highest CMCellulase activity of 75.84 μg/ml/min for crude enzyme of T. mentagrophyte heat-pretreated rice husk. The duration of 96 h gave maximum activity of 58.21 μg/ml/min for crude enzyme of T. soudanense heat-pretreated rice husk. Highest CMCellulase activities of 67.02 μg/ml/min and 69.02 μg/ml/min at pH of 5 were recorded for crude enzymes of monocultures of T. soudanense (TS) and T. mentagrophyte (TM) heat-pretreated rice husk. Heating before treatment with T. mentagrophyte gave 44.50 ± 10.90 cellulose as maximum, total lignin value of 28.90 ± 1.80 from co-culture of T. soudanense and T. mentagrophyte (TS + TM), hemicellulose content of 30.50 ± 2.12 (% ± SEM) from pre-heated rice husk treated with T. soudanense (TS), carbohydrate content of 16.79 ± 9.14, and reducing and non-reducing sugar values of 2.66 ± 0.45 and 14.13 ± 8.69 were all obtained from for pre- heated rice husk treated with T. mentagrophyte (TM). The pre-heated rice husk treated with T. mentagrophyte (TM) fermented with palm wine yeast gave bioethanol value of 11.11 ± 0.21 (% ± standard deviation) as the highest yield.