کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
---|---|---|---|---|
1355871 | 981070 | 2012 | 4 صفحه PDF | دانلود رایگان |
عنوان انگلیسی مقاله ISI
Cellular uptake of covalent conjugates of oligonucleotide with membrane-modifying peptide, peptaibol
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کلمات کلیدی
موضوعات مرتبط
مهندسی و علوم پایه
شیمی
شیمی آلی
پیش نمایش صفحه اول مقاله
![عکس صفحه اول مقاله: Cellular uptake of covalent conjugates of oligonucleotide with membrane-modifying peptide, peptaibol Cellular uptake of covalent conjugates of oligonucleotide with membrane-modifying peptide, peptaibol](/preview/png/1355871.png)
چکیده انگلیسی
Using the membrane-modifying peptide, trichorovin-XIIa (TV-XIIa), which is an 11-residual peptaibol isolated from the fungus Trichoderma viride, we synthesized covalent conjugates of 20-mer oligonucleotide with TV-XIIa to examine the potential use of TV-XIIa in cellular delivery. The results indicated that the conjugates were progressively taken up by human lung carcinoma A549 cells. Next, the antisense effects of the conjugates on p53 protein expression were examined. The p53 expression was significantly decreased by ca. 20–50% in the presence of the conjugates upon treatment with the transfection solution at the concentration of 5 μM.
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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry - Volume 20, Issue 10, 15 May 2012, Pages 3219–3222
Journal: Bioorganic & Medicinal Chemistry - Volume 20, Issue 10, 15 May 2012, Pages 3219–3222
نویسندگان
Shun-ichi Wada, Yasunari Hitora, Saori Yokoe, Osamu Nakagawa, Hidehito Urata,