کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1355976 981080 2011 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
A ribonucleopeptide module for effective conversion of an RNA aptamer to a fluorescent sensor
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
A ribonucleopeptide module for effective conversion of an RNA aptamer to a fluorescent sensor
چکیده انگلیسی

Ribonucleopeptide (RNP) is a new class of scaffold for modular fluorescent sensors. We report here a short RNA motif that induces an efficient communication between the structural changes associated with the ligand-binding event of RNA aptamer and an optical response of a fluorescent RNP module. An optimized short RNA motif was used as a communication module for the rational design of modular RNP sensors. A modular combination of a GTP-binding RNA aptamer, the short RNA motif and the fluorophore-labeled RNP module afforded a fluorescent GTP sensor that retain the ligand-binding affinity of the parent aptamer.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry - Volume 19, Issue 19, 1 October 2011, Pages 5771–5775
نویسندگان
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