Binding of 14-3-3 proteins to a single stranded oligodeoxynucleotide aptamer

A synthetic library of ca. 1013 single stranded oligodeoxynucleotides, each comprising a randomized 40mer sequence and homogeneous 10mer flanking regions, was screened for binding to recombinant human 14-3-3γ. A single aptamer, which showed similar affinities (KD ∼ 10−8 M) for six isoforms of the protein, has been shown to bind to undenatured 14-3-3 protein in the cerebral spinal fluid of scrapie infected sheep.

We describe identification of a synthetic ssDNA aptamer which binds to undenatured 14-3-3 proteins and its application to the affinity precipitation of 14-3-3 from the cerebral spinal fluid of a scrapie infected sheep.