کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1357060 | 981194 | 2006 | 10 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: High-mannose-type glycan modifications of dihydrofolate reductase using glycan–methotrexate conjugates High-mannose-type glycan modifications of dihydrofolate reductase using glycan–methotrexate conjugates](/preview/png/1357060.png)
Various high-mannose-type glycan modifications of dihydrofolate reductase (DHFR) were achieved by ligand-based approach using glycan–methotrexate (MTX) conjugates as tight binding glycan bearing ligands for DHFR. The resulting glycan–MTX conjugates and the corresponding artificial glycoproteins could be useful as oligosaccharide- and glycoprotein-probes to perform quantitative analysis of glycan recognizing protein such as lectins, glycosyltransferases or glycosidases. Moreover, artificial glycoproteins having two different high-mannose-type glycans were developed for the first time by a combination of two different types of glycan modification strategies.
Artificial glycoproteins having structurally defined synthetic oligosaccharides were generated.Figure optionsDownload as PowerPoint slide
Journal: Bioorganic & Medicinal Chemistry - Volume 14, Issue 15, 1 August 2006, Pages 5220–5229