کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1359115 981384 2010 4 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
The ODN probes conjugating the Cu(II) complex enhance the luminol chemiluminescence by assembling on the DNA template
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
The ODN probes conjugating the Cu(II) complex enhance the luminol chemiluminescence by assembling on the DNA template
چکیده انگلیسی

Potent peroxidase-like activity of the β-ketoenamine (1)–dicopper (II) complex (2) for the chemiluminescence (CL) of luminol either in the presence or absence of H2O2 has been previously demonstrated by our group. In this study, the β-ketoenamine (1) as the ligand unit for copper(II) was incorporated into the oligonucleotide (ODN) probes. It has been shown that the catalytic activity of the ODN probes conjugating the ligand–Cu(II) complex is activated by hybridization with the target DNA with the complementary sequence. Thus, this study has successfully demonstrated the basic concept for the sensitive detection of nucleic acids by CL based on the template-inductive activation of the catalytic unit for CL.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry - Volume 18, Issue 24, 15 December 2010, Pages 8614–8617
نویسندگان
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