کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1359402 | 981402 | 2009 | 8 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: Preparation of conophylline affinity nano-beads and identification of a target protein Preparation of conophylline affinity nano-beads and identification of a target protein](/preview/png/1359402.png)
Conophylline, a vinca alkaloid extracted from the tropical plant Ervatamia microphylla, has been shown to induce the differentiation of insulin-producing β-cells in cultured cells and in animals. However, its mechanism of action and the molecular target have remained unclear. Therefore, we prepared a fishing probe with conophylline to identify the target protein by using latex nano-beads, which are newly innovated tools for affinity-purification. With these conophylline-linked nano-beads, we found that conophylline directly interacted with ARL6IP. ARL6IP may thus be involved in the mechanism of cellular differentiation of β-cells, and this probe should be useful to find other target proteins.
We prepared conophylline-linked nano-beads and conophylline was found to directly interact with ARL6IP.Figure optionsDownload as PowerPoint slide
Journal: Bioorganic & Medicinal Chemistry - Volume 17, Issue 17, 1 September 2009, Pages 6188–6195