کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1359827 981417 2009 7 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Characterization of emodin metabolites in Raji cells by LC–APCI-MS/MS
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Characterization of emodin metabolites in Raji cells by LC–APCI-MS/MS
چکیده انگلیسی

A rapid, simple, and sensitive liquid chromatography–atmospheric pressure chemical ionization tandem mass spectrometry (LC–APCI-MS/MS) method was developed for the identification and quantification of emodin metabolites in Raji cells, using aloe-emodin as an internal standard. Analyses were performed on an LC system employing a Cosmosil 5C18 AR-II column and a stepwise gradient elution with methanol–20 mM ammonium formate at a flow rate of 1.0 mL/min operating in the negative ion mode. As a result, the starting material emodin and its five metabolites were detected by analyzing extracts of Raji cells that had been cultivated in the presence of emodin. The identification of the metabolites and elucidation of their structures were performed by comparing their retention times and spectral patterns with those of synthetic samples. In addition to the major metabolite 8-O-methylemodin, four other metabolites were assigned as ω-hydroxyemodin, 3-O-methyl-ω-hydroxyemodin, 3-O-methylemodin (physcion), and chrysophanol.

In addition to the major metabolite 8-O-methyl emodin, ω-hydroxyemodin, 3-O-methyl-ω-hydroxyemodin, 3-O-methylemodin, and chrysophanol were detected as emodin metabolites by LC–APCI-MS/MS method in Raji cells.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry - Volume 17, Issue 21, 1 November 2009, Pages 7493–7499
نویسندگان
, , , , , , ,