Electronic detection of DNA mutation based on strand exchange reaction

A new approach to electronic detection of a single base mismatch is described. The assay involves the electrochemical measurements of DNA strand exchange reactions (SERs) between electrode-bound redox-modified DNA duplex and target DNA, where the sequence of redox-modified DNA is exchangeable to that of the target DNA. The presence of a single base mismatch can be determined from the slower SER rates compared with fully matched DNA.

Electronic detection of DNA mutation based on DNA strand exchange reactions (SERs) is described.The presence of a single base mismatch can be electrochemically determined from the slower SER rates compared with fully matched DNA.Figure optionsDownload as PowerPoint slide