Identification of antibacterial and antiviral activities of novel fused 1,2,4-triazine esters

Biological activities of the designed novel fused 1,2,4-triazine esters (3a, 3d-j) are reported. In particular, heterobicycles 3d and 3e revealed comparable antibacterial potencies in vitro to that of ampicillin. Compounds 3e, 3g and 3j exhibited antiviral activities against the selected viruses' DNA (human adenovirus type 5-Ad-5) or/and RNA (human enterovirus Echo-9). Simultaneously, their cytotoxicities towards the normal HEK-293 and GMK cells were established. In particular, heterobicycle 3j, non-toxic for GMK cells, was found to exhibit virucidal properties against Echo-9 virus justifying its further investigation as the potential antiviral agent.