کد مقاله | کد نشریه | سال انتشار | مقاله انگلیسی | نسخه تمام متن |
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1361591 | 981467 | 2007 | 6 صفحه PDF | دانلود رایگان |
![عکس صفحه اول مقاله: New amino and acetamido monomethine cyanine dyes for the detection of DNA in agarose gels New amino and acetamido monomethine cyanine dyes for the detection of DNA in agarose gels](/preview/png/1361591.png)
Some new monomethine cyanine dyes derived from quinoline and benzothiazole have been prepared and characterized by 1H and 13C NMR, FTIR, FABHRMS, and visible spectroscopy. The dyes containing amino and acetamido groups were conveniently synthesized by the condensation of two p-toluenesulfonate heterocyclic quaternary salts and were obtained in the forms of iodide, bromide, and tosylate counteranions. These dyes were compared to ethidium bromide as stains for DNA in electrophoretic gels. The overall results obtained for the sensitivity of these dyes suggest the suitability of acetamido moiety over the amine one and bromide as the counteranion when compared with iodide and tosylate, with a similar capacity of DNA detection in relation to the ethidium bromide stain over the concentration range of 1–3 ng.
Five new monomethine cyanine dyes have been prepared and spectroscopically characterized. The acetamido dyes present to be promising tools for DNA analysis at low levels of concentration when UV light is required for visualization, overcoming the use of the mutagenic EtBr stain or radioactivity for detecting low levels of DNA.Figure optionsDownload as PowerPoint slide
Journal: Bioorganic & Medicinal Chemistry - Volume 15, Issue 16, 15 August 2007, Pages 5537–5542