Pyrene–perylene as a FRET pair coupled to the N2′-functionality of 2′-amino-LNA

Detection of nucleic acid hybridization via fluorescence resonance energy transfer (FRET) using pyren-1-ylmethyl and perylen-3-ylmethyl N2′-functionalized 2′-amino-LNA nucleosides incorporated into oligonucleotides exhibited a clear distance dependence of the FRET efficiency, ranging from below 10% when the fluorophores were approximately 40 Å apart to approximately 90% when the fluorophores were in close proximity.

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