کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1362156 981479 2010 9 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Synthesis and ribonuclease A inhibition activity of resorcinol and phloroglucinol derivatives of catechin and epicatechin: Importance of hydroxyl groups
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Synthesis and ribonuclease A inhibition activity of resorcinol and phloroglucinol derivatives of catechin and epicatechin: Importance of hydroxyl groups
چکیده انگلیسی

The reported ribonuclease A inhibitory activity of the green tea extracts prompted us to synthesize novel catechin/epicatechin based conjugates with resorcinol and phloroglucinol with the aim to increase the number of phenolic OH groups. These are found to be more effective inhibitors of ribonuclease A as compared to catechin and epicatechin thus indicating the importance of number of phenolic OH groups for the inhibition of ribonucleolytic activity. Fluorescence studies have been carried out to evaluate the binding parameters. The protein–ligand docking studies are also performed to gain insight into the protein–polyphenols interactions. The epicatechin based polyphenols 1 and 2 also showed inhibition of angiogenin-induced angiogenesis, as determined by chorioallantoic membrane (CAM) assay.

(A) Lane1: RNA, Lane 2: RNase A + RNA, Lane 3: RNaseA + Epicatechin + RNA, Lane 4: RNase A + 2 + RNA, Lane 5: RNase A + 1 + RNA, Lane 6: RNase A + 3′-CMP + RNA. (B) Lane 1: RNA, Lane 2: RNase A + RNA, Lane 3: RNase A + Catechin + RNA, Lane 4: RNase A + 3 + RNA, Lane 5: RNase A + 4 + RNA.Figure optionsDownload as PowerPoint slide

ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry - Volume 18, Issue 17, 1 September 2010, Pages 6538–6546
نویسندگان
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