Reduction of nonspecific binding proteins to self-assembled monolayer on gold surface

We developed a gold coated glass chip bearing a poly(ethyleneglycol) (PEG) type compound as hydrophilic spacer for surface plasmon resonance studies, which enabled adequate estimation of Kd value between FK506 and FKBP12 not only using purified FKBP12 (Kd = 22 nM) but also using Escherichia coli lysate expressing FKBP12 (Kd = 15 nM). These results indicated effectiveness of the PEG spacer for reduction of nonspecific interactions. Chemical stability and simple surface-structure of the novel chip are also attractive.

Application of 1 on gold surface enabled adequate estimation of Kd value between FK506 and FKBP12 by SPR not only using purified FKBP12 but using Escherichia coli lysate expressing FKBP12.Figure optionsDownload as PowerPoint slide