کد مقاله کد نشریه سال انتشار مقاله انگلیسی نسخه تمام متن
1364598 981540 2008 5 صفحه PDF دانلود رایگان
عنوان انگلیسی مقاله ISI
Chemical probes for profiling fatty acid-associated proteins in living cells
موضوعات مرتبط
مهندسی و علوم پایه شیمی شیمی آلی
پیش نمایش صفحه اول مقاله
Chemical probes for profiling fatty acid-associated proteins in living cells
چکیده انگلیسی

Chemical probes appended with reactive electrophiles afford powerful tools for profiling discrete protein families in living cells. Herein, we have synthesized cell-permeable chemical probes that target fatty acid-associated proteins. These fatty acid-based chemical probes contain acyloxymethylketone or fluorophosphonate functional groups and an alkyne click chemistry tag for visualization of covalently modified proteins by in-gel fluorescence scanning. Our fatty acid-based chemical probe affords new tools to evaluate the activity/expression of lipid-associated proteins that should facilitate their functional characterization and inhibitor discovery.

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ناشر
Database: Elsevier - ScienceDirect (ساینس دایرکت)
Journal: Bioorganic & Medicinal Chemistry Letters - Volume 18, Issue 22, 15 November 2008, Pages 5982–5986
نویسندگان
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